《Table 1 Optimum culture medium for dorsal root ganglion-satellite glial cells》

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《A novel primary culture method for high-purity satellite glial cells derived from rat dorsal root ganglion》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

DMEM/F12:Dulbecco’s modified Eagle’s medium/nutrient mixture F-12；B27:B-27supplement；PSS:penicillin-streptomycin solution；BSA:bovine serum albumin；NRG1-β1:neuregulin 1-β1；T3:triiodothyronine 3；T4:triiodothyronine 4.

Neonatal rats were sterilized with 75%alcohol（disinfection time>3 minutes）.Rats were decapitated and sacrificed.The skin，muscles，and ribs were cut to obtain the spine，which was cooled on ice in Dulbecco’s modified Eagle’s medium/nutrient mixture F12 Ham（DMEM/F12，1:1 mixture）（C11330500BT；Gibco Life Technologies Inc.，Grand Island，NY，USA） .The spinal muscles were removed，and then both sides of spinous processes cut using ophthalmic scissors.The spinal cavity was exposed and emptied.Additionally，blood vessels and nerve fibers in the spinal cavity were completely removed under a stereomicroscope.The DRG was then clamped under a stereomicroscope，followed by gentle removal of residual nerve fibers and capsules with fiber clamps（Figure 1A）.Treated DRGs were collected in 35 mm Petri dishes on ice with DMEM/F12（1:1）containing penicillin/streptomycin.A sufficient number of DRGs（approximately54）were collected in 35 mm dishes and then seeded into 6-well plates containing SGC medium（500μL per well）（Table 1） .All operations were performed on ice.Cells were cultured in a humidified 5%CO2 incubator at 37°C（Figure 1B）.