《Table 6 THC and DHC (×10 6 cells/mL) of M.rosenbergii fed with control, B-Zn and Zn-NPs supplemente

《Table 6 THC and DHC (×10 6 cells/mL) of M.rosenbergii fed with control, B-Zn and Zn-NPs supplemente   提示:宽带有限、当前游客访问压缩模式
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《Influence of two different dietary zinc sources in freshwater prawn Macrobrachium rosenbergii post larvae》


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B-Zn:bulk zinc;Zn-NPs:zinc nanoparticles;1 B-Zn and Zn-NPs free feed;n=3(3 samples from each treatment),mean±SD;mean values within the same row sharing the same superscript are not signifi cantly diff erent(P>0.05);THC:total haemocytes count;DHC:diff

Experimental feeds were prepared using locally available feed ingredients(Table 1).Feed ingredients were purchased from local shops.For preparation of these experimental feeds,fi shmeal and soybean meal,wheat fl our and tapioca fl our,and cod liver oil were used as protein,carbohydrate and lipid sources respectively.Also,tapioca fl our and egg albumin were used as binding agents,and vitamins(B complex with vitamin C)and Zn free mineral mix were also added.The optimum concentrations(60 mg/kg)of dietary B-Zn and Zn-NPs were selected for M.rosenbergii PL according to previous reports of Muralisankar et al.(2014,2015a).In the present study,99.99%pure B-Zn(size 10μm)and Zn-NPs(size 50 nm)were purchased from Sigma-Aldrich Chemicals Pvt.Limited,Bangalore,India.These two diff erent sources of Zn were supplemented in the basal feeds at the concentration of 60 mg/kg separately.Feed without supplementation of Zn source was served as control.According to the method of Muralisankar et al.(2014),about 3.0±0.84 mm dia feeds were prepared and stored in plastic containers until used for feeding experiments.