《Table 1–Study of photoreactivation under different light conditions.》

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《Visible-light-driven photocatalytic inactivation of bacteriophage f2 by Cu-TiO_2 nanofibers in the presence of humic acid》

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A large number of studies have reported that there is a type of reactivation enzyme in E.coli，which can repair damaged DNAafter the E.coli is deactivated by UV irradiation，thereby restoring the activity of the bacteria.This phenomenon is called photoreactivation（Li et al.，2017）.Because of the existence of photoreactivation，traditional UV disinfection technology faces great risks to microbial safety.Therefore，in order to investigate the reliability of the photocatalytic antiviral technique，it is necessary to determine whether photoreactivation takes place in our experimental system.As shown in Table 1，in the absence of HA，bacteriophage f2 was completely deactivated after 2 hr，and then the reaction solution was placed under different light conditions.The virus concentration was still 0 PFU/mL after 2 hr and 4 hr.Similarly，in the presence of HA，the virus concentration remained at the magnitude of 104PFU/mL.Therefore，it could be concluded that the phenomenon of photoreactivation did not occur in this photocatalytic antiviral system.This might be because bacteriophage f2 itself does not possess a reactivation enzyme like E.coli.Generally，photocatalytic disinfection by Cu-TiO2could deactivate bacteriophage f2 rapidly without photoreactivation，exhibiting low microbial risk and relatively high reliability.