《Table 1–General description of the sampling events.》

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《One-year survey of opportunistic premise plumbing pathogens and free-living amoebae in the tap-water of one northern city of China》

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The locations with asterisk（*）represented the taps of high-use-frequency.

All the collected tap water samples were cold water sampled at the same time of day during normal operation and usage.A total of 11 sampling sites dispersed across the city were monitored（Table 1）.Four sampling events（Autumn:Sep，2015；Winter:Dec，2015；Spring:Apr，2016；Summer:Jul，2016）were performed.The taps were wiped with 75%ethanol and run for15 sec prior to water collection（Donohue et al.，2015）.At each tap，3 L of water was sampled in three 1-L sterile glass media bottles，respectively.1 L of water was used for the testing of water quality parameters and the other 2 L of water（bottles containing sterile sodium thiosulfate）was used for DNA extraction（Appendix A Fig.S2）.All the water samples for DNA extraction were stored at4°C and transported to the lab and processed within 6–12 hr.