《Table 6 Modifying effect of diabetes mellitus on GST genotypes in relation to the risk of POAG》

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《GSTM1-null and GSTT1-active genotypes as risk determinants of primary open angle glaucoma among smokers》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

OR:Odds ratio adjusted for age and gender.

Sample Collection and DNA Analysis Commercially available DNA kit（Qiagen，USA）was used to isolate DNA from blood leucocytes.The analysis of GSTM1 and GSTT1polymorphisms was carried out by polymerase chain reaction（PCR）using the multiplex PCR method[28].CYP1A1 was used as a housekeeping gene（Table 1）.PCR protocol was as followed:denaturation at 94℃for 4min followed by94℃for 30s；annealing:59℃for 30s；extension:72℃for45s；number of cycles:30；final extension:72℃for 5min.The blinded samples for quality control were included to ensure the validation of procedures and steps in the genotype identification and the investigators who performed the multiplex PCR analysis were uninformed of the case-control status.The similarity of the results for the blinded samples was100%.