《Table 2 Grading standards of cold tolerances in bitter gourd at bud stage and seedling stage》

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《Identification and Evaluation of Cold Tolerance in Bitter Gourd (Momordica charantia L.) at Bud and Seedling Stages under Different Temperature Conditions》

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The experiment was carried out in the solar greenhouse of the eight team of Cucurbits and Vegetables Laboratory，Tropical Crop Germplasm Research Institute.Full seeds of bitter gourd with uniform size were selected and subjected to pregermination.After the emergence of white buds，the seeds were sown into 50-hole trays filled with Peilei medium（Zhenjiang Peilei Organic Fertilizer Co.，Ltd.）for raising seedlings.At the stage of two leaves and one heart，uniform seedlings were selected and cultured in an illumination incubator for 2 d under the day/night temperature at 28℃/18℃，light period of 14 h and dark period of 10 h，and illumination intensity of 6 000 lx.The seedlings were then placed in an manual climatic box（Shanghai Yiheng Scientific Instrument Co.，Ltd.，MGC-450HP-2）for low temperature treatment.During the period of treatment，the cold injury characteristics were investigated，and the cold damage symptoms are shown in Table 1.