《Tab.1 Sequences of the single guide RNAs (g RNAs) of the target genes》

《Tab.1 Sequences of the single guide RNAs (g RNAs) of the target genes》   提示:宽带有限、当前游客访问压缩模式
本系列图表出处文件名:随高清版一同展现
《利用CRISPR/Cas9技术建立NOD/SCID/IL2Rγ~(-/-)免疫缺陷小鼠(英文)》


  1. 获取 高清版本忘记账户?点击这里登录
  1. 下载图表忘记账户?点击这里登录

The g RNAs were designed using the online software(http://www.genome-engineering.org).The complementary oligos for each target sequence were heated at 95℃for5 min,annealed,and the short double-strand DNA fragments were ligated into p T7-Bbs I-g RNA vector.The sequence of g RNAs is listed in Tab.1.