《Table S2.Data of heteropteran species downloaded from GenBank used in assembling sequences.》

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《Biased heteroplasmy within the mitogenomic sequences of Gigantometra gigas revealed by sanger and high-throughput methods》

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For the single individual of G.gigas investigated for the impact of different sequencing methods，total genomic DNA extracts were divided into six parts.Three of them were sequenced with the HTS platform by China National GeneBank（BGI-Shenzhen，China），and the other three were used for regular PCR and then sequenced by Sanger method.For each copy of the genomic DNA extracts latterly used for Sanger sequencing，the overlapped short fragments were separately amplified，sequenced，and manually assembled to provide three independent results of sequencing the complete mitogenome and nrDNA cluster（Sanger et al.，1977）.The clone with pEASY-T3（a TA cloning vector）was used in the sequencing of complete sequences of cox1，and 25 different clones were selected randomly and sequenced，respectively.These fragments were amplified using perfectly matched primers（Table S1）.The PCR amplification was carried out in a 50-μL reaction system containing 6μL 10×LA PCR Buffer II（Mg2+Plus），6μL of d NTP mixture（2.5 m M），0.5μL LA Taq（TaKaRa Biotechnology，Dalian，China），2μL of each primer（10μM），2μL DNA template and 31.5μL distilled water.The thermal cycling program of the PCR consisted of an initial denaturation at 94°C for 2 min，35 thermal cycles（94°C for 30 s，43–52°C for 30 s，72°C for 1 min）and a final extension at 72°C for 8 min.Colony PCR was carried out in a 25-μL reaction volume followed by 30 amplification cycles.