《Table 3 Recovery for the assay of 11 BAs》

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《天然牛黄、体外培育牛黄和人工牛黄中胆汁酸类成分及其在人肠源Caco-2细胞模型中吸收转运的对比研究（英文）》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

Analysis was performed using a Waters Xevo TQMS system（Waters，Milford，MA，USA）.Masslynx software（version 4.1）was used for data acquisition and processing.The analytical column was an Acquity UPLC HSS T3（2.1×100 mm；1.8μm）（Waters） maintained at 40°C.The mobile phases were constitutive of 10 mmol/L ammonium acetate in aqueous（A）and a mixture of acetonitrile and methanol（3:1）（B） ，both of which contained 0.1%（v/v）formic acid.The linear gradient was set as follows:2%B at 0 min，15%B at 1 min，40%B at4 min，60%B at 10 min，95%B at 14 min，95%Bat 22 min and 2%B at 22.5 min for 5 min of equilibration.The flow rate was set at 0.3 mL/min.The auto-sampler was fixed at 4°C and the injection volume was 10μL per analysis.Electrospray ionization was performed in negative ion mode.The mass spectrometer（MS）source parameters were set as follows:source temperature 500°C，nebulizer gas（nit rogen）flow rate of 7.0 L/min，capillary voltage of 2.0 kV，cone voltage of 42 V，and radio frequency voltage at 0.2 V.The dwell time was 10 ms.Analysis was performed in multiple reaction monitoring（MRM）mode.The MRM of each compound was predetermined by MS/MS analysis（Table 1）.