《Table 1–Coded and actual values of variables in central-composite design for the preparation of Lop

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《"Systematically optimized topical delivery system for Loperamide hydrochloride: Formulation design,in vitro and in vivo biopharmaceutical evaluation"》

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The selected independent variables were the EtOH:DGME ratio（X1）and EtOH concentration（X2）.The dependent variables were flux（Y1）and skin retention（Y2）.The independent and dependent variable with coded levels，low（-1），medium（0）and high（+1）levels were listed in Table 1.Table 4 summarized an account of the 13 experimental runs studies.Twodimensional contour plots and three-dimensional response surface plots were also developed.