《Table 7 Single-factor analysis of melanoma metastasis》

《Table 7 Single-factor analysis of melanoma metastasis》   提示:宽带有限、当前游客访问压缩模式
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《Expression and predictive value of miR-489 and miR-21 in melanoma metastasis》


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Main reagents and instruments:Trizol reagent(American Applied Invitrogen company),qRT-PCR kit and minScript reverse transcription kit(Dalian TaKaRa company),HBS-1096A microplate reader(Nanjing Detie Experimental Equipment Co.,Ltd.),and real-time quantitative PCR instrument(American BioRad company)were used.The sequences of primers,miRNA negative controls,miR-489,miR-21,and internal reference U6 were designed and synthesized by Shanghai Gemma company.More details are shown in Table 1.