《Table 3 Frequency distribution of the number of di-and tri-nucleotide motif repeat units》

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《基于转录组测序的葛根SSR标记研究与利用（英文）》

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Initially，the 229SSRs were scored for amplicon size polymorphism in six Puerariacollections.We found that 28SSRs gave highly polymorphic and bands after PCR amplification.Fig 3is shown the allelic variation of PtSSR144in some P.lobata resources.These markers were used to determine the polymorphic information content（PIC）values and to estimate the genetic similarity of the 44 Puerariagermplasm collections（Table 4）.A total of90bands were amplified using the 28SSRs in the44accessions，consisting of 89alleles which gave a polymorphism rate 98.9%.The average number of alleles per SSR locus was 3.178 6.The PIC of the28markers ranged from 0.083 0to 0.774 2with average of 0.455 7.When PIC>50%，the marker was highly polymorphic；when 25%