《Table 1 Proliferation situation of axillary buds of Platycodon grandiflorus》

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《Study on Tissue Culture and Rapid Propagation of Platycodon grandiflorus》

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Note:In the table，MS was used as the basic medium，to which certain kinds of hormones and 30 g/L sucrose were added.Proliferation coefficient=number of pro-liferation buds/number of inoculated buds.The growth situation of proliferation buds were divided i

After 40 days of proliferation culture，the number of the proliferating buds varied somewhat between different plant hormones and different concentrations of media.According to Table 1，the proliferation coefficient obtained by using BA as a mitogen was significantly higher than that of KT.Seen from the growth situation of the regeneration buds，the regeneration buds induced by BA were stronger.However，the regeneration buds induced by KT grew slowly，and there existed vitrification phenomenon，showing that KT was not conducive to the proliferation culture of P.grandiflorus.There were also differences between different concentrations of the same mitogen in terms of proliferation situation of P.grandiflorus.When BA was used as mitogen，the proliferation coefficient increased firstly and then reduced with the increase of its concentration.As KT concentration increased to 0.5 mg/L，the regeneration buds grew fastest，and the proliferation coefficient was the highest.The results indicate that the proliferation effect of P.grandiflorus was the best under the action of suitable BA.To sum up，the MS medium containing 0.5mg/L BA，0.5 mg/L IAA and 30 g/L sucrose was the fittest for the proliferation culture of P.grandiflorus.