《Table 4 Correlation between total circulating tumor cell status and clinicopathological factors of

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《Molecular detection of epithelial-mesenchymal transition markers in circulating tumor cells from pancreatic cancer patients:Potential role in clinical practice》

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CTC:Circulating tumor cells.

CTC subpopulations were identified using a multiplex RNA-ISH assay.Four epithelial biomarkers[epithelial cell adhesion molecule（EpCAM）and cytokeratin（CK）8/18/19]，two mesenchymal biomarkers（vimentin and twist），and a leukocyte biomarker，leukocyte common antigen（CD45），were applied to capture and characterize the CTCs.The hybridization assay was performed as previously described[22].The assay was performed in a 24-well plate（Corning，NY，United States），and the cells on the membrane were treated with protease（Qiagen，Hilden，Germany）and subsequently subjected to serial hybridization reactions with capture probes that were specific for the intended examined genes（Supplementary Table 1）.We applied4′，6-diamindino-2-phenylindole（DAPI）to stain the nuclei，and the cells were analyzed with a fluorescence microscope.