《Table 1 Temperature, oxygen, total dissolved solids (TDS) , pH and Chl a mean values (SD) in agricu

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《Does wetland watershed land use influence amphibian larval development A relevant effect of agriculture on biota》

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At the end of the experimental period，60metamorphs（15 metamorphs from each experimental pool）were humanely euthanized in 150 to 200 mg/L e.g.MS-222（buff ered with sodium bicarbonate to achieve pH 7.0）and were frozen at-80°C for biochemical analysis.Before being analysed，all the individuals were weighed quickly in pre-cooled Eppendorf tubes，using a microbalance（Mettler Toledo microbalance；precision 0.01 mg）and kept in crushed ice until homogenization.The lipid peroxidation was quantifi ed with the concentration of the malondialdehyde（MDA），an end product of lipid peroxidation，following the method proposed by Esterbauer and co-workers（Esterbauer et al.，1991）.The standard curve preparation was carried out with10 mmol/L TMP（1，1，3，3-tetramethoxypropane；Sigma-Aldrich）at 4°C.Protein content was determined colorimetrically using the Bradford method（Bradford，1976）with Coomassie Brilliant Blue G-250（Sigma-Aldrich）using bovine serum albumin as a standard.