《Table 2 Effect of different types and concentrations of auxins combined with 0.1 mg/L TDZ on shoot

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《卡琪花蒂玛茎段增殖及生物反应器培养（英文）》

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Z means seperation within columns by Duncan’s multiple range test at 5%level.NS:nonsignificant；**:significant at P≤0.01；***:significant at P≤0.001.

To study the synergistic effect of auxins and cytokinins，the node explants were cultured on MS medium fortified with different type of auxins（IBA，IAA and NAA）at the concentrations of0.1，0.5 and 1.0 mg/L and supplemented with 0.1mg/L TDZ or 5.0 mg/L zeatin.When 0.1 mg/L TDZ was combined or mixed with auxins in the medium，the number of shoots increased compared with that of the TDZ treatment，alongside with a slight shoot elongation.The IBA and IAA were more effective for shoot propagation than the NAA（Table 2，Fig.3）.The largest number of shoots（22 shoots per explant）was obtained at 0.1 mg/L TDZ and 0.1 mg/L IBA.The proportion of auxin to cytokinin was a determinant for meristem formation and the hormone balance established by growth regulators determined the type of buds induced[17，22].The inherent endogenous auxin and cytokinin levels must have also played a role in the observed data[23].The TDZ，a derivative of phenylurea with cytokininlike activity，was found to be able to induce thebiosynthesis of cytokinin，inhibit the degradation of endogenous auxin and stimulate the de novo synthesis of auxins[23-26].Therefore，the low-level TDZ combined with the low-level IBA may result in the higher number of shoots in this study.