《Table 1–The characteristics of nanoemulsions.》

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《The accelerated blood clearance phenomenon of PEGylated nanoemulsion upon cross administration with nanoemulsions modified with polyglycerin》

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The oil phase containing DiR，MCTs，E80 and PG610-DSPE/m PEG2000-DSPE/PGC was dissolved at 55°C.Sterile water was quickly injected into this oil phase with stirring for 10 min at55°C in a water bath to obtain the primary emulsions.The final emulsions were obtained by using a laboratory ultrasonic cell pulverizer（JY92-II，Ningbo Scientz Biotechnology，Zhejiang，China）at 200 W for 2 min and at 400 W for 6 min，respectively.The final emulsions were extruded through polycarbonate membranes with a pore size of 0.22μm respectively，and were made isotonic with 50%（m/v）glucose（Shandong Yuwang industry，China）.The nanoemulsions modified with 1 mol%，3 mol%or9 mol%PG610-DSPE were named as 1%GE，3%GE 9%GE respectively.The nanoemulsion modified with 9 mol%m PEG2000-DSPE was named as 9%PE.The nanoemulsion modified with 1 mol%PGC was named as 1%HE.The nanoemulsions，1%GE，3%GE 9%GE，9%PE and 1%HE，were used as detection nanocarriers for containing Di R in the formulations.The empty nanoemulsions for the first injection in repeated injection regime were prepared using the similar formulations without DiR.These empty nanoemulsions were named as 1%GE-B，3%GE-B，9%GE-B，9%PE-B and 1%HE-B.The particle size distribution and Zeta potential were determined by dynamic light scattering using a Nicomp380 HPL submicron particle analyzer（Particle Sizing Systems，Santa Barbara，CA，USA）and the results are shown in the Table 1.