《Table 3 Hazard gradation》

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《Toxic Effects of Micro-plastics on Zebrafish Embryos》

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Exposure experiment of zebrafish embryos0.6-1.0μm polystyrene particles were used for the exposure experiment.The four experiment concentrations designed according to the preliminary experiment were 1 000，250，25 and 2.5 mg/L，respectively.Each group included five parallel samples.The specific operation was the same as the preliminary experiment.In the experimental process，the mortality and hatchability of the embryos were observed by semistatic method.When replacing the toxic liquid，coagulated eggs were also removed，and the development status of zebrafish embryos was observed under a microscope.The lethal indicators were egg coagulation，no beginning of gastrulae differentiation，no termination of gastrulae differentiation，no somite，no tail extension，no heartbeat and no hatching.The teratogenic indicators included changed body length，cyrtosis，malformed tail，and changed heart rate.For the computation of heart rate，9 embryos were randomly selected from the four replicates of each treatment to carry out 1 min of cardiac impulse statistics.The observation indicators of zebrafish embryos at various exposure time were shown in Table 1.