《分子生物学 影印版》

Section A-Cells and macromolecules1

A1Cellular classification1

A2 Subcellular organelles4

A3 Macromolecules7

A4 Large macromolecular assemblies11

Section B-Protein structure15

B1Amino acids15

B2 Protein structure and function18

B3 Protein analysis25

Section C-Properties of nucleic acids31

C1Nucleic acid structure31

C2 Chemical and physical properties of nucleic acids36

C3 Spectroscopic and thermal properties of nucleic acids40

C4 DNA supercoiling43

Section D-Prokaryotic and eukaryotic chromosome structure47

D1Prokaryotic chromosome structure47

D2 Chromatin structure49

D3 Eukaryotic chromosome structure54

D4 Genome complexity59

Section E-DNA replication63

E1DNA replication:an overview63

E2 Bacterial DNA replication68

E3 Eukaryotic DNA replication72

Section F-DNA damage,repair and recombination77

F1Mutagenesis77

F2 DNA damage81

F3 DNA repair84

F4 Recombination87

Section G-Gene manipulation91

G1DNA cloning:an overview91

G2 Preparation of plasmid DNA96

G3 Restriction enzymes and electrophoresis99

G4 Ligation,transformation and analysis of recombinants104

Section H-Cloning vectors111

H1Design of plasmid vectors111

H2 Bacteriophage vectors115

H3 Cosmids and YACs120

H4 Eukaryotic vectors124

Section I-Gene libraries and screening129

I1Genomic libraries129

I2 cDNA libraries132

I3 Screening procedures137

Section J-Analysis and uses of cloned DNA141

J1Characterization of clones141

J2 Nucleic acid sequencing146

J3 Polymerase chain reaction150

J4 Organization of cloned genes154

J5 Mutagenesis of cloned genes158

J6 Applications of cloning162

Section K-Transcription in prokaryotes165

K1Basic principles of transcription165

K2 Escherichia coli RNA polymerase168

K3 The E.coliσ70 promoter170

K4 Transcription initiation,elongation and termination173

Section L-Regulation of transcription in prokaryotes179

L1The lac operon179

L2 The trp operon183

L3 Transcriptional regulation by alternativeσfactors187

Section M-Transcription in eukaryotes191

M1The three RNA polymerases:characterizationand function191

M2 RNA Pol Ⅰgenes:the ribosomal repeat193

M3 RNA Pol Ⅲ genes:5S and tRNA transcription197

M4 RNA Pol Ⅱ genes:promoters and enhancers201

M5 General transcription factors and RNA Pol Ⅱ initiation203

Section N-Regulation of transcription in eukaryotes207

N1Eukaryotic transcription factors207

N2 Examples of transcriptional regulation213

Section O-RNA processing and RNPs219

O1rRNA processing and ribosomes219

O2 tRNA processing,RNase P and ribozymes225

O3 mRNA processing,hnRNPs and snRNPs228

O4 Alternative mRNA processing234

Section P-The genetic code and tRNA237

P1The genetic code237

P2 tRNA structure and function241

Section Q-Protein synthesis247

Q1Aspects of protein synthesis247

Q2 Mechanism of protein synthesis251

Q3 Initiation in eukaryotes257

Q4 Translational control and post-translational events261

Section R-Bacteriophages and eukaryotic viruses265

R1Introduction to viruses265

R2 Bacteriophages269

R3 DNA viruses274

R4 RNA viruses278

Section S-Tumor viruses and oncogenesb283

S1Oncogenes found in tumor viruses283

S2 Categories of oncogenes287

S3 Tumor suppressor genes290

Further reading295

Index299