《Biological Applications of Freezing and Drying》求取 ⇩
作者 | R.J.C.Harris 编者 |
---|---|
出版 | Academic Press Inc. |
参考页数 | 415 ✅ 真实服务 非骗流量 ❤️ |
出版时间 | 1954(求助前请核对) 目录预览 |
ISBN号 | 无 — 违规投诉 / 求助条款 |
PDF编号 | 818143058(学习资料 勿作它用) |
求助格式 | 扫描PDF(若分多册发行,每次仅能受理1册) |
Chapter 1Effects of Low Temperatures on Living Cells and Tissues&BY AUDREY U.SMITH1
Ⅰ.Introduction1
Ⅱ.Thermal Shock6
Ⅲ.Removal of Water11
Ⅳ.Temperature Gradients17
Ⅴ.Extra- and Intracellular Changes:Crystallization and Vitrification22
Ⅵ.The Action of Glycerol and Related Substances31
Ⅶ.Duration of Survival at Low Temperatures:Metabolic and Physical Factors42
Ⅷ.Effects of Cold on the Whole Animal45
Ⅸ.Practical Applications48
References50
Chapter 2The Development of Freeze-Drying&BY E.W.FLOSDORF63
Ⅰ.General Considerations63
Ⅱ.Equipment Developments66
Ⅲ.Application Developments70
References83
Chapter 3Theoretical Aspects of Drying by Vacuum Sublimation&BY R.I.N.GREAVES87
Ⅰ.Introduction87
Ⅱ.Freezing90
Ⅲ.Primary Drying100
Ⅳ.Secondary Drying and Packaging123
Ⅴ.Some Difficulties125
References126
Chapter 4The Preservation of Blood Plasma and Blood Products by Freezing and Drying&BY MAX M.STRUMIA129
Ⅰ.General Considerations129
Ⅱ.Preparation of Plasma131
Ⅲ.Freezing of Plasma134
Ⅳ.Drying of Plasma from the Frozen State137
Ⅴ.Conditions and Details of Design Related to Removal of Water137
Ⅵ.An Apparatus for Freeze-Drying Plasma142
Ⅶ.Stability of Plasma in the Dry State147
Ⅷ.Freeze-Drying of Other Blood Products147
References148
Chapter 5The Freeze-Drying of Antibiotics&BY J.H.SINGER151
Ⅰ.Introduction151
Ⅱ.Drying in Individual Containers152
Ⅲ.Equipment for Large-Scale Drying of Penicillin in Individual Containers155
Ⅳ.Bulk Drying169
References175
Chapter 6The Freeze-Drying of Mother's Milk&BY G.G.A.MASTENBROEK177
Ⅰ.Introduction177
Ⅱ.Collection of the Milk178
Ⅲ.The Drying Plant179
Ⅳ.Properties and Advantages of Dried Milk181
Ⅴ.Conclusions182
References183
Chapter 7The Freeze-Drying of Foodstuffs&BY R.GANE185
Ⅰ.Introduction185
Ⅱ.Freeze-Drying Equipment188
Ⅲ.General Conclusions190
References190
Chapter 8The Preservation of Media for the Culture of Bacteria and Tissues&BY DUNCAN C.HETHERINGTON193
Ⅰ.Introduction193
Ⅱ.Frozen-Dried Tissue Culture Media194
Ⅲ.Commercial Production of Media195
References199
Chapter 9The Preservation of Viruses&BY R.J.C.HARRIS201
Ⅰ.Introduction201
Ⅱ.Optimum Suspending Media for Viruses202
Ⅲ.Stability of Viruses to Freezing203
Ⅳ.Drying Frozen Virus Suspensions by Vacuum Sublimation204
Ⅴ.Storage of Dried Virus Preparation209
Ⅵ.General Considerations210
References211
Chapter 10The Preservation of Bacteria&BY R.M.FRY215
Ⅰ.Introduction215
Ⅱ.Estimation of Survival216
Ⅲ.Technique of Drying Bacteria217
Ⅳ.Factors Influencing Survival231
Ⅴ.The Death of Dried Bacteria247
Ⅵ.Further Work249
References250
Chapter 11The Preservation of Tissues&BY R.E.BILLINGHAM253
Ⅰ.Introduction253
Ⅱ.Studies on Preserved Nerve Grafts255
Ⅲ.A Method of Effecting Nerve Reunion with Frozen-Dried Artery Segments258
Ⅳ.The Preservation of Corneal Tissue259
Ⅴ.The Preservation of Blood Vessel Grafts262
Ⅵ.Studies on Frozen and on Frozen-Dried Tumor Tissues264
Ⅶ.Studies on the Freezing,Freeze-Drying,and Storage of Mammalian Skin270
Ⅷ.The Possible Application of Frozen-Dried Tissues in the Investigation of Problems of Morphogenesis277
Ⅸ.Conclusions278
References280
Chapter 12The Effects of Residual Moisture in Frozen-Dried Materials,and Its Measurement&BY L.G.BECKETT285
Ⅰ.Introduction285
Ⅱ.Effects of Residual Moisture on Non-Viable Materials286
Ⅲ.Effects of Residual Moisture on Frozen-Dried Viruses and Bacteria287
Ⅳ.Methods of Moisture Determination289
Ⅴ.Conclusion300
References300
Chapter 13.The Application of Freeze-Drying to Electron Microscopy&BY ROBLEY C.WILLIAMS303
Ⅰ.Introduction:General Remarks303
Ⅱ.Freeze-Drying for Electron Microscopy:General Considerations307
Ⅲ.Earlier Methods of Freeze-Drying Electron Microscope Specimens308
Ⅳ.Description of a New Freeze-Drying Method309
Ⅴ.Critical Examination of Earlier Freeze-Drying Methods312
Ⅵ.Evaluation of the New Freeze-Drying Method313
Ⅶ.Difficulties in the Electron Microscopy of Frozen-Dried Materials316
Ⅷ.Examples of Electron Micrographs of Frozen-Dried Specimens318
Ⅸ.Summary326
References327
Chapter 14Freezing and Drying of Tissues for Morphological and Histochemical Studies&BY ISIDORE GERSH AND JOHN L.STEPHENSON329
Ⅰ.Introduction330
Ⅱ.Theory of Freezing333
Ⅲ.Tissue Observations335
Ⅳ.Description of the Drying Process338
Ⅴ.Factors Controlling the Rate of Drying341
Ⅵ.Quantitative Relations of the Factors Controlling the Rate of Drying345
Ⅶ.Efficiency of Drying Apparatus348
Ⅷ.Design of Drying Apparatus350
Ⅸ.Preparation of Dried Tissue for Sectioning360
Ⅹ.Morphology and Histochemistry of Frozen-Dried Tissues361
References380
AUTHOR INDEX385
SUBJECT INDEX395
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