《Table 1 Primer sequences for quantitative real-time PCR amplification》

《Table 1 Primer sequences for quantitative real-time PCR amplification》   提示：宽带有限、当前游客访问压缩模式
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《Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut(Arachis hypogaea L.)seedlings under continuous cropping》


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1) Au.N7IXY and Au.GJ0WT represent RAM1 and RAM2 ID from Arachis duranesis which is thought to be one of the diploid ancestor，respectively.

Total RNA was isolated from the roots，and cDNA was synthesized for qRT-PCR analyses using SYBR Premix Ex Taq Polymerase（TaKaRa，Japan）according to the manufacturer’s protocol.The selected genes were analyzed using a Bio-Rad i Q1 Real-Time PCR machine（Bio-Rad，USA）.The primers are shown in Table 1.The control reactions were conducted using primers Tua5-F and Tua5-R，which were reported by Chi et al.（2012）.At least three replicates were tested per sample.Relative mRNA（fold）differences were assessed with the 2–ΔΔCt formula（Livak and Schmittgen 2001）.

图表编号XD0047433800    严禁用于非法目的
绘制时间2019.02.20
作者CUI Li、GUO Feng、ZHANG Jia-lei、YANG Sha、MENG Jing-jing、GENG Yun、WANG Quan、LI Xin-guo、WAN Shu-bo
绘制单位Biotechnology Research Center, Shandong Academy of Agricultural Sciences、Key Laboratory of Crop Genetic Improvement and Ecological Physiology of Shandong Province、Scientific Observing and Experimental Station of Crop Cultivation in East China, Ministry of
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