《Table 2 Effect of sucrose concentration in culture on grafting survival rate》

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《Studies on Factors Affecting Survival Rate of Micro-grafting in Vitro in Persimmon》

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Sucrose as carbon source could provide energy for plant cells，and also could induce the formation of phloem.Navarro et al.found during micro-grafting of citrus that sucrose concentration plays a key role in survival of micro-grafted plants.With D.lotus as stock，Jiro was grafted and cultured to medium with different sucrose concentrations.The results（Table 2）showed that with the increase of sucrose concentration，the grafting survival rate increased significantly，but there were no significant difference between the sucrose concentrations of 5%and 7%，and when the sucrose concentration was 7%，the base parts of tissue culture plantlets browned seriously，which might be due to that the improvement of sugar content improved osmotic pressure in medium.Comprehensively from the experimental results and experimental cost，the sucrose concentration of 5%was more suitable.