《第五届国际转基因动物学术研讨会论文集》求取 ⇩

SECTION 1TRANSGENIC ANIMALS AND MAMMARY GLAND BIOREACTOR（转基因动物与乳腺生物反应器）1

0101 CONSTRUCTSYNTHETICGENEENCODINGARTIFICIALSPIDERDRAGLINESILKPROTEINANDITS EXPRESSION IN MILK OF TRANSGENIC MICEXu Hong-Tao,Fan Bao-Liang,Yu Shu-yang等1

0102 SUPERTRANSGENIC CARP:PREPARATION OF GYNOGENETIC OFFSPRING AND ANALYSIS OF GENOTYPIC PURITYLING Li-Qun(梁利群)，YAN Xue-Chun(闫学春)，SUN Xiao-Wen(孙效文)等6

0103 THE ESTABLISHMENT OF TRANSGENIC MICE OF HUMAN PROTEIN C GENES AND THE GENE'S EXPRESSION IN MAMMARY GLAND（人蛋白C转基因小鼠的建立及乳腺表达）ZHAO Bao-quan(赵宝全)，ZHANG Jin-Gang(章金刚)等9

0104 EXPRESSION OF TRANSGENIC MICE CARRYING GREEN FLUORESCENT PROTEIN GENE（绿色荧光蛋白基因（GFP）在转基因小鼠体内的表达）XU Yi-mei(徐艺玫)，LIU Li-Jun(刘丽均)等12

0105 THE ESTABLISHMENT AND APPLICATION OF THE NUCLEAR TRANSPLANTATION OF MAMMALIAN EGG CELL—NEW RESEARCH ON THE TECHNIQUE OF HUMAN EMBRYONIC STEM CELLAND TRANSGENIC ANIMAL（哺乳动物卵细胞核移植技术的建立及应用—人类胚胎干细胞及转基因动物技术新探）XIONG Ya-Long(熊亚隆)，NA Dong Quan(纳冬荃)，YUE Yu-Han(岳玉罕)15

0106 GENE PACKED USING PEI IN COMBINATION WITH ELECTROPORATION FACILITATE THE EXOGENOUS DNA ENTRY INTO THE NUCLEUS OF MOUSE SPERM（阳离子多聚物PEI结合电穿孔实现小鼠精子的核内转染的研究）HUANG Wu-Jian（黄吴键），YUAN Jin（袁进）等18

0107 EXPERIMENTAL STUDY ON GENERATION OF TRANSGENIC MICE BY COMBINATION OF INTRATESTICULAR INJECTION WITH IN VIVO ELECTROPORATION（睾丸内注射和体内电穿孔联合法建立转基因小鼠的实验研究）YUAN Jin（袁进），AN Jing(安靓)等22

0108 PRODUCTION OF TRANSGENIC MICE CARRYINGHUMAN P16INK4A BY MICROINJECTION（用显微注射法制备携带人P16基因的转基因小鼠的初步研究）ZHANG Shou-Chun（张守纯），YU Ye-Hui（于业辉）等27

0109 STUDIES ON THE MECHANISM OF THE STABLEEXPRESSION OF EXOGENOUS GENE IN MAMMARY GLANDS（乳腺持续表达外源基因的机理研究）AN Jing(安靓)，LI Jin（李进）等29

0110 ESTABLISHMENT OF TRANSGENIC MOUSE MODEL WITH LYMPHOMAGUO Chang-Zhan，ZHOU Wei-Min等31

0111 ANIMAL CLONING AND TRANSGENIC MAMMARY GLAND BIOREACTORS（动物克隆与转基因动物乳腺生物反应器）Cheng Guo-Xiang（成国祥）32

0112 VISUALIZING TUMOR DEVELOPMENT AND GENE EXPRESSION IN VIVO THROUGH BIOLUMINESCENCE IMAGINGNing Zhang32

0113 GENERATION OF MT-PA TRANSGENIC GOAT BY SPERM-MEDIATED GENE TRANSFER（利用精子载体方法生产MT-PA转基因山羊）叶华虎，刘珠果，卢建申等33

0114 APRELIMINARY STUDY ON GENERATION OF TRANSGENIC ANIMALS BY INTRA-OVARY INJECTION（卵巢注射法制备转基因动物的初步研究）刘维全，王吉贵，李全等33

0115 BIOLOGICAL CHARACTERISTICS OF THE TRANSGENIC MOUSE HARBORING HBV COMPLETE GENOMIC DNA（乙型肝炎病毒全基因组转基因小鼠的生物学特性）ZI Xiao-Yuan(訾晓渊)，ZHANG Nan（张南）等34

0116 CRUCIAL ROLE OF GENE ORDER IN THE DEVELOPMENTALLY REGULATED EXPRESSION MODE OF HUMAN α-GLOBIN GENE CLUSTERWANG Yi，WANG Zhao等36

0117 BOTHHS-40 AND HS-4～33 ARE SIGNIFICANT FOR THE REGULATION OF THEHUMAN α-LIKE GLOBIN GENE EXPRESSIONS ON THE WHOLE CLUSTER LEVELTANG Xiao-Bin，FENG Dong-Xiao等37

0118 EXPLORING THE FUNCTION OF PSEUDOGENES ON THE HUMAN ALPHA GLOBIN CLUSTERZHAO Wang，KE Xi-Song等37

0119 CREATING TRANSGENIC MODEL OF CARDIAC DISEASES WITH C57BL/6J MICEZHUO Ming-Lei，LIU Guang等38

0120 MEASUREMENT OF BLOOD PRESSURE O FTRANSGENIC C57BL/6J MICE MODEL OF CARDIAC DISEASESSUN Li-Hong,ZHOU Ming-Lei等38

0121 CONSTRUCTION OF RED FLUORESCENCE PROTEIN TRANSGENIC WHITE CLOUD MOUNTAIN MINNOWJIAN Qing,BAI Jun-Jie等39

0122ANIMAL MODEL FOR REAL-TIME MEASUREMENTS OF NEURONAL ACTIVITY IN TRANSGENIC MICEShi-Liang Shen,Allsion Xu等40

0123 INDUCIBLE REPORTER AND HIV NEF GENE EXPRESSION IN CD4+CELLS OF TRANSGENIC MICEJisen Huai,Gabi Niedenmann40

0124 OVEREXPRESSION OF GLY56/GLY80/GLY81-MUTANT INSULIN-LIKE GROWTH FACTOR（IGF）BINDING PROTEIN-3（IGFBP-3）IN TRANSGENIC MICEGUI Yao-Ting,Josef V.Silha等41

0125 HIGH EXPRESSION OF HUMAN ERYTHROPOIETIN IN MAMMARY GLAND OF TRANSGENIC GOATSCHEN Jian-Quan,WU Guo-Xiang等42

0126 EXPRESSION OF AN ENGINEERED FORM OF HUMAN TISSUE-TYPE PLASMINOGEN ACTIVATOR IN MOUSE MILKSHA Hong-Ying（沙红英），LIU Si-Guo（刘思国）等42

0127 EFFICIENT GENERATION OF TRANSGENIC GOATS BY NUCLEAR TRANSFERYU Guo-Hhua(余国华),WU Guo-Xiang（吴国祥）等43

0128 TRANSFER OF AN EXPRESSION YAC INTO GOAT FETAL FIBROBLASTS BY CELL FUSION FOR MAMMARY GLAND BIOREACTORZHANG Xu-Ffeng,WU Guo-Xiang等43

0129 THE CHARACTERIZATION AND BIOLOGIC ACTIVITY OF RECOMBINANT HUMAN ERYTHROPOIETIN IN GOAT MILKZHAO Jian-Yang，CHEN Jian-Quan等44

0130 GENERATION OF BOVINE FCRN ALPHA CHAIN HARBORING BAC TRANSGENIC MICEBender B.,Bodrogi L.等44

0131 IMPROVING EFFICIENCY OF TRANSGENIC RAT PRODUCTIONThomas L.Saunders,Wanda E.Filipiak45

0132 TRANSGENIC RABBITS AS MODELS FOR PRODUCING BIOLOGICALLY ACTIVE HUMAN RECOMBINANT PROTEINSHiripi L.,Baranyi M.等46

0133 ESTABLISHMENT OF DOUBLE TRANSGENIC MICE MODEL SPATIOTEMPORALLY EXPRESSING HCV-C GENE MEDIATED BY TET-ON SYSTEMCHEN Xi-Gu，YANG Guo-Shu等47

0134 RE-STARVATION OF NUCLEUS-DONATING CELLS FOR GENE TRANSFER IN DAIRY GOATS ON EMBRYONIC DEVELOPMENT AFTER NUCLEAR TRANSPLANTATION（奶山羊转基因供核细胞的再饥饿对核移植胚胎发育的影响）陈建泉，张爱民邓47

0135 HUMAN PAPILLOMAVIRUS TRANSGENIC ANIMAL MODEL:STRATEGIES STILL TO BE IMPROVED（人乳头瘤病毒转基因动物模型：方法仍待改进）49

0136 ESTABLISHMENT OF HIGH-COPY HBV TRANSGENIC MICE AND ITS APPLICATION（高复制HBV转基因小鼠建立及应用研究概况）刘光泽，顾为望等51

0137 SOMATIC CLONING AND MAMMARY GLAND BIOREACTOR（体细胞克隆与动物乳腺生物反应器）SHI Qiao-Ting(施巧婷),XIN Xiao-Ling(辛晓玲)等55

0138 PROSPECT OF THE ESTABLISHMENT OF TRANSGENIC ANIMALS BY PRIMITIVE SPERMATOGONIUM TRANSPLANTATION（用精原干细胞移植技术制作转基因动物的研究前景）吴应积，罗奋华，旭日干59

SECTION 2GENE TARGETING（GENE KONCKOUT）（基因打靶／基因敲除）62

0201 FSH,LH,E2 AND PIN BLOOD SERUM OF FMR1 KNOCKOUT MOUSE AND FVB MOUSE（雌性FMR1基因敲除小鼠血清生殖激素的比较研究）DAI Li-Jun（戴丽军），LIAO Jun（廖军）等62

0202 REGULATION OF DOPAMINE RECEPTORS ON COCAINE-INDUCED MAPK SIGNALING PATHWAY AND C-FOS GENE EXPRESSION（多巴胺受体在可卡因诱导的MAPK通路激活和C-FOS基因表达中的作用）Liu Nu-Yun(刘怒云),Zhang Lu(张璐)等65

0203 GENERATION OF THE CONDITIONAL GENE TARGETED MOUSE MODELS FOR HUMAN DISEASESYANG Xiao70

0204 GENE TARGETING WITH C57BL/6 STRAINJingsong Chu，Illya Bronshteyn等70

0205 MEKK1 REGULATES F-ACTIN AND EPITHELIUM MOVEMENTLIU Nu-Yun,Zhang Lin等71

0206 PRODUCTION OF KAPPA-CASEIN KNOCK-OUT ES CELL LINE DERIVED CHIMERASL.Hiripil,M.Baranyil等72

0207 PRELIMINARY STUDY ON EFFECTS OF PASG KNOCKOUT ON BRAIN DEVELOPMENT AND RELEVANT GENE EXPRESSION（PASG基因敲除对脑发育及其相关基因表达影响的初步研究）ZHANG Quan-Bin(张全斌),DONG Jun(董军)等72

0208 OBSERVATIONAL STUDY ON BLEOMYCIN-INDUCED EXPRESSION OF A-ACTIN IN SMOOTH MUSCLE IN PULMONARY INTERSTITIAL FIBROSIS IN SMA-CRE/ROSA26 TRANSGENIC MICE（用SMA-CRE/ROSA26转基因鼠来观察博莱霉素诱导的肺间质纤维化中A平滑肌肌动蛋白的表达）吴壮，蔡琳，徐军73

0209 MOLECULAR AND CELLULAR BASIS OF DRUG ADDICTION（制备诱导性基因敲除小鼠研究安非他明依赖机制）LI Sheng-Bin(李生斌)74

SECTION 3　RNA INTERFERENCE（RNA干扰）78

0301RNA INTERFERENCE OF EXOGENOUS GFP GENE EXPRESSION IN NEURO-2A CELLSZHANG Xiao-Qin，LI Feng等78

0302 SCREENING AND IDENTIFICATION OF SIRNA TO SUPPRESS MACROPHAGE MIGRATION INHIBITORY FACTOR（MIF）GENE EXPRESSION（抑制人巨噬细胞移动抑制因子（MIF）表达的SIRNA的筛选和鉴定）SHAN Zhi-Xin（单志新），LIN Qiu-Xiong（林秋雄）等81

0303 FABRICATION OF AN OLIGONUCLEOTIDE ARRAY FOR GENOME-WIDE MICRORNA AND EXPRESSION PROFILE OF MICRORNA IN HUMAN CANCER CELLSMA Zhuo-Ya,LIU Min等87

0304 GROWTH REPRESSION OF HUMAN CERVICAL CANCER HELA CELLS BY SIRNA TARGETING HUMAN TELOMERASE CATALYTIC SUBUNIT（HTERT）MRNALI Xin,SHEN Zhi-Wei等87

0305 DOWN-REGULATION OF ALPHA-FETOPROTEIN（AFP）IN HEPG2 CELLS INTERFERED WITH SPECIFIC SIRNAAND ITS IMPACT ON THE CELLULAR FUNCTIONTANG Xiao-Yan，LI Xin等88

0306 MIRNA:BIOGENESIS AND FUNCTIONTANG Hua88

SECTION 4EMBRYO ENGINEERING AND NUCLEAR TRANSFER（胚胎工程，核移植）90

0401 IN VITRO DEVELOPMENT OF RECONSTRUCTED EMBRYO BY NUCLEAR TRANSFER WITH PORCINE SOMATIC CELLS（猪体细胞核移植重构胚的体外发育）ZHENG De-Fu（张德福），Wang Y Ying（王英）90

0402 PRODUCTION OF SHEEP TRANSGENIC EMBRYOS DERIVED FROM TRANSFECTED SOMATIC CELLS WITH HOMOLOGOUS RECOMBINANT VECTORS（利用同源重组载体生产转基因体细胞克隆绵羊胚胎）AO Hong（敖红），PAN Qiu-Zhen（潘求真）等95

0403 REMOVAL OF NECROTIC BLASTOMERES FROM HUMAN CRYOPRESERVED EMBRYOS ON PREGNANCY OUTCOMELIU Wei-Xin，LUO Meng-Jun等100

0404 INTEGRATION AND EXPRESSION OF DOUBLE SCREENING MARKER VECTOR IN DAIRY GOAT FETAL FIBROBLASTS（双元选择标记载体在奶山羊胎儿成纤维细胞的整合与表达）李文蓉，谭立新等106

0405 SCAVENGE STUDY OF THE FRESH EMBRYO ON EMBRYO TRANSFER IN SPECIFIC MUTANT MICE（采用鲜胚对特种突变小鼠进行胚胎移植的净化研究）李瑞生，仇志华等111

0406 CRYOPRESERVED MORULAE CAN BE USED TO EFFICIENTLY GENERATE GERMLINE-TRANSMITTING CHIMERAS BY BLASTOCYST INJECTIONJanice V.Parker-Thomburg,Jennifer L.Alana等113

0407 STEM CELL AND THERAPEUTIC CLONINGZHOU Qi114

0408 EFFECTS OF DIFFERENT CHEMICAL ACTIVATION PROTOCOLS ON EARLY PARTHENOGENETIC DEVELOPMENT OF PORCINE OOCYTESLU Sheng-Sheng，WEI Feng等114

0409 IN VITRO PRODUCTION OF CATTLE EMBRYOS DERIVED FROM ABATTOIR OVARIESZHANG De-Fu，WANG Ying等115

0410 STUDY ON THE OVUM PICK-UP BY ULTRASOUND-GUIDED RETRIEVAL AND IN VITRO EMBRYO PRODUCTION IN BOVINE（奶牛活体采卵体外生产胚胎的研究）WANG Yu-Hong（王玉红），CHEN Jing-Bo（陈静波）等115

0411 STUDY ON THE TECHNIQUE OF VITRIFICATION OF PORCINE OOCYTESZHANG De-Fu，LIU Dong等117

0412 PROGRESS ON THE STUDY OF DIFFERENTIAL MEMBRANE PROTEINS BETWEEN X\Y CHROMOSOME BEARING SPERMATOZOA IN MAMMAL（哺乳动物X、Y精子间差异膜蛋白的研究进展）WANG Dong（王栋），CHENG Jin-Hua（程金华）等117

0413 GENETIC ENGINEERED ANIMALS AND CONTEMPORARY CCOMPARATIVE MEDICINE（基因工程动物与现代比较医学研究）顾为望 刘光泽 王万山121

SECTION 5STEM CELLS（干细胞）128

0501 THE ESTABLISHMENT OF PTET-ON INTEGRATED EMBRYONIC STEM CELL LINE OF MICE（稳定整合PTET-ON载体小鼠胚胎干细胞株的建立）ZHANG Mei-Ying（张梅英），LI Hua（李华）等128

0502 INDUCTION AND DIFFERENTIATION OF HEPATIC STEM CELLS INTO INSULIN-PRODUCING CELLS（肝干细胞诱导分化为胰岛素分泌细胞的实验研究）AN Jing（安靓），SUN Bing（孙冰）等133

0503 STEM CELLS ANDAIDS（干细胞技术与艾滋病研究）XIONG Ya-Long（熊亚隆）136

0504 TRANSCRIPTOMIC ANALYSIS OF THE CHICKEN EMBRYONIC STEM CELLS（CESC）B.Pain136

0505 IN VITRO ISOLATION,CULTIV-ATION AND IDENTIFICATION OF FETAL HEPATIC STEM CELLS（胎肝干细胞的分离、培养与鉴定）SUN Bing（孙冰），AN Jing（安靓）137

0506 IN UTERO TRANSPLANTATION OF HUMAN CORD BLOOD PRIMITIVE CELLS TRANSDUCED WITH MIG VECTOR INTO FETAL GOATS TO INVESTIGATE THE LONG-TERM GROWTH POTENTIAL AND PLASTICITY OF STEM CELLSHUANG Shu-Zhen，ZENG Yi-Tao138

0507 REPROGRAMMING SOMATIC CELL IN VITRO:NEW STRATEGY FOR ESTABLISHMENT OF PLURIPOTENT STEM CELLLINES（体细胞体外重编程：建立多能干细胞系的新策略）LI Yu-Qiang(李裕强)139

0508 BONE MARROW MESENCHYMAL STEM CELLS AND MYOCARDIAL TISSUE ENGINEERING（骨髓间充质干细胞与心肌组织工程）YU Xi-Yong（余细勇），LI Xiao-Hong（李晓红）144

SECTION 6NEW ADVANCES IN TECHNOLOGY（新技术，新近展）149

0601 CONSTRUCTION AND IDENTIFICATION OF HUMAN TISSUE KALLIKREIN GENE EUKARYOTIC EXPRESSING VECTORDAI Yong（戴勇）149

0602 MURINE RESIDENT BONE MARROW MACROPHAGE PROMOTE ENUCLEATION OF TERMINALLY DIFFERENTIATED ERYTHROLEUKEMIA CELLS THROUGH DIRECT ATTACHMENT AND PHAGOCYTOSISKANG Jie，LIU Qian等152

0603 STUDY OF THE GENE EXPRESSION PROFILE ON PIGR-TRANSFECTED TMNE CELLS TREATED WITH EBV AND TPA COOPERATIVELY（EBV与TPA协同处理转染人PIGR的TMNE细胞基因表达谱的研究）WANG Shuang（王爽），LV Li-Chun（吕丽春）156

0604 DETECTION OF GENETICCALLY MODIFIED COMPONENT IN FAMILIAR EDULIS FUNGI BY MULTIPLEX PCR（食用菌中转基因成分的多重PCR检测技术研究）陈文炳，江树勋等160

0605 STUDY ON THE IMMOBILIZATION OF SSDNA ON THE PT ELECTRODE（单链DNA在PT电极上的固定化研究）YANG Min-Li（杨敏丽）163

0606 ROLE OF VEGFR-3 SIGNALING IN TUMOR LYMPHANGIOGENESIS AND LYMPHATIC METASTASISYulong He，Iiro Rajantie等167

0607 INHIBITORY EFFECT OF CARVEDILOL ON CALCIUM CURRENT IN RAT VENTRICULAR MYOCYTES（卡维地洛对大鼠心室肌细胞L-型钙通道的抑制作用研究）DENG Chun-Yu（邓春玉），YU Xi-Yong（余细勇）168

0608 CHROMOSOMAL ANALYSIS OF BOVINE BLASTOCYSTS PRODUCED BY ICSI AND IVF WITH SORTED SPERMSZHANG Ming，LU Yang-Qing等171

0609 RAPID HIGH-THROUGHPUT GENERATION OF PRECISE CUSTOM GENE MODIFICATIONS IN MICE USING THE VELOCIGENE SUITE OF TECHNOLOGIESNicholas W.Gale，Andrew J.Murphy等172

0610 DEVELOPMENT-RELATED GENE EXPRESSION IN MOUSE PREIMPLANTATION EMBRYOS WITH DIFFERENT DEVELOPMENTAL POTENTIAL（不同发育潜力小鼠植入前胚胎中发育相关基因的表达）LI Xiang-Ping，Y.Kato，Y.Tsunoda等173

0611 APPLICATION AND PROSPECTS OF PARTICLE-MEDIATED GENE TRANSFER IN CANCER THERAPY（微粒介导的基因转移在癌症治疗中应用前景）CHEN Jian-Quan（陈建海），PING Yuan（平渊）173

0612 ADVANCES IN MAMMALIAN GENOMIC IMPRINTING（哺乳动物印迹基因研究进展）Jiang MS(江明生),杨小淦等174

0613 DEVELOPMENT OF GENE TRANSFECTION INSTRUMENT AND ITS APPLICATIONIN ANIMAL CELLS（基因脉冲导入仪的研制及在动物转基因技术上的应用）SU Ming-Hao（苏明皓），XU Bao-Qiang（徐宝强）等176

APPENDIX:Author Index179