《Forensic DNA profiling protocols》

1 Recovery of High-Molecular-Weight DNA from Blood and Forensic SpecimensPeter M. Schneider1

2 Recovery of DNA for PCR Amplification from Blood and Forensic Samples Using a Chelating ResinJeanne M. Willard, Dennis A. Lee, and Mitchell M. Holland9

3 PCR Analysis of DNA from Fresh and Decomposed Bodies and Skeletal Remains in Medicolegal Death InvestigationsManfred N. Hochmeister19

4 PCR Analysis from Cigaret Butts, Postage Stamps, Envelope Sealing Flaps, and Other Saliva-Stained MaterialManfred N. Hochmeister, Oskar Rudin, and Edda Ambach27

5 Quantification of DNA by Slot-Blot AnalysisJulia Andersen33

6 Rapid Assessment of PCR Product Quality and Quantity by Capillary ElectrophoresisJohn M. Butler39

7 Southern Blotting of Genomic DNA for DNA ProfilingJim Thomson49

8 Preparation and Use of Alkaline-Phosphatase-Conjugated Oligonucleotide Probes for Single-Locus and Multilocus VNTR AnalysisWayne P. Childs, Michael B. T. Webb, and Gillian Rysiecki57

9 Preparation and Use of 32P-Labeled Single-Locus VNTR Probes in Identity TestingJurgen Henke and Lotte Henke69

10 Statistical Methods Employed in Evaluation of Single-Locus Probe Results in Criminal Identity CasesBruce S. Weir83

11 Interpretation and Statistical Evaluation of Multilocus DNA Fingerprints in Paternity and Relationship TestingGillian Rysiecki and John F. Y. Brookfield97

12 Overview of PCR-Based Systems in Identity TestingBernd Brinkmann105

13 An Introduction to PCR Primer Design and Optimization of Amplification ReactionsJames M. Robertson and Jennifer Walsh-Weller121

14 Analysis of Amplified Fragment-Length Polymorphisms (VNTR/STR Loci) for Human Identity TestingBruce Budowle and Robert C. Allen155

15 Amplification of Short Tandem Repeat Loci Using PCRNiels Morling173

16 Manual Electrophoretic Methods for Genotyping Amplified STR LociChris P. Phillips, Angel Carrecedo, and Maviky V. Lareu181

17 Genotyping STR Loci Using an Automated DNA SequencerDavid Watts193

18 Separation of PCR Fragments by Means of Direct Blotting ElectrophoresisLotte Henke and J?rgen Henke209

19 Amplification and Sequencing of Mitochondrial DNA in Forensic CaseworkRobert J. Steighner and Mitchell Holland213

20 Minisatellite Variant Repeat Unit Mapping Using PCR (MVR-PCR)David L. Neil225

21 Sex Determination by PCR Analysis of the X-Y Amelogenin GeneAtsushi Akane245

22 Species Determination by Analysis of the Cytochrome b GeneRita Barallon251

23 Use of the AmpliType PM + HLA DQA1 PCR Amplification and Typing Kits for Identity TestingMichael L. Baird261

24 The Use of Capillary Electrophoresis in Genotyping STR LociJohn M. Butler279

25 Solid-Phase Minisequencing as a Tool to Detect DNA PolymorphismAnn-Christine Syvanen291

Index299