《Table 1Gene-specific primers used for q PCR.》

《Table 1Gene-specific primers used for q PCR.》   提示：宽带有限、当前游客访问压缩模式
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《Hydrogen-rich saline protects against hepatic injury induced by ischemia-reperfusion and laparoscopic hepatectomy in swine》


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HGF:hepatocyte growth factor；TGF-β:transforming growth factor-beta；TNF-α:tumor necrosis factor alpha；IL-1β:interleukin-1 beta；IL-6:interleukin-6；IL-10:interleukin-10.

Total RNA was isolated from livers using the TRIzol reagent according to the manufacturer’s instructions（Invitrogen，Shanghai，China）.First-strand c DNA was synthesized using the ReverTra Ace q PCR RT Master Mix（Toyobo，Osaka，Japan）.Quantitative real-time PCR was performed on a LightCycler 480 Real-Time PCR System（Roche Applied Science，Penzberg，Germany）in a 10-μL final reaction volume containing 5μL FastStart Universal SYBR Green Master（Roche Applied Science，Penzberg，Germany），1μL c DNA，0.3μL of each primer and 3.4μL ddH 2 O.The PCR program was performed as follows:95°C for 15 s；40 cycles of 95°C for 15 s，60°C for 60 s，and 60°C for 30 s.Primers used in the present study were synthesized by Sangon Biotech（Shanghai，China）（Table 1） .

图表编号XD0031654000    严禁用于非法目的
绘制时间2019.02.15
作者Yan-Song Ge、Qian-Zhen Zhang、Hui Li、Ge Bai、Zhi-Hui Jiao、Hong-Bin Wang
绘制单位College of Veterinary Medicine, Northeast Agricultural University、College of Veterinary Medicine, Northeast Agricultural University、College of Veterinary Medicine, Northeast Agricultural University、College of Veterinary Medicine, Northeast Agricultural Un
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