《Table 1–cis-regulatory elements involved in endosperm expression.》

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《"Imprinting, methylation, and expression characterization of the maize ETHYLENE-INSENSITIVE 2-like gene"》

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The sequences of the gene and its predicted promoter region were downloaded from http://www.maizesequence.org/index.html.The gene was amplified from B73 using KOD FX DNA polymerase（Toyobo，Osaka，Japan）.Rapid amplification of cDNA ends was used to determine the transcriptional start site（TSS）and 3′untranslated region（UTR）of EIN2-like.Cis-regulatory elements were predicted by Plant CARE（http://bioinformatics.psb.ugent.be/webtools/plantcare/html/）[32]and Cp G islands were predicted by the EMBOSS Cpgplot program（http://www.ebi.ac.uk/Tools/seqstats/emboss＿cpgplot/）.Multiple sequence alignments were performed using Clustal X1.83，as implemented by MEGA 5.0，on full-length proteins[33].Phylogenetic trees were constructed by neighbor joining with the JTT model and Complete Deletion[33].Reliability values at each branch point represent bootstrap cores（1000 replicates）.Conserved motifs of the EIN2 protein sequences were identified by MEME（http://meme-suite.org/tools/meme）.The sequences of all the primers used in the study are listed in Table S1.