《Table 2–Comparison of molecular weight (MW) fractions of non-proteinaceous and proteinaceous COM of

《Table 2–Comparison of molecular weight (MW) fractions of non-proteinaceous and proteinaceous COM of   提示:宽带有限、当前游客访问压缩模式
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《Investigating the coagulation of non-proteinaceous algal organic matter: Optimizing coagulation performance and identification of removal mechanisms》


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Non-proteinaceousCOM was isolated in two steps from the cells of Chlorella vulgaris,i.e.,isolation of water-soluble COM from the cells and isolation of the non-proteinaceous fraction from water-soluble COM.To provide insight into the composition of original and isolated fractions,the carbohydrate,protein and lipid content in the total COM(cells including both water-soluble and insoluble components),water-soluble COM(extracted from cells)and NM(extracted from water-soluble COM)was quantified as detailed in Table 1.It can be seen that the portion of proteins and especially lipids differed in the total and dissolved COM biomass of C.vulgaris.This is given by the fact that some proteins and most lipids are insoluble in water.According to the literature,protein content in C.vulgaris dry biomass varies with growth conditions 4%–40%(Becker,1994;Laurens et al.,2014;Safi et al.,2014;Yeh and Chang 2012).Some of the protein is bound to cell walls(about20%)and the membranes of organelles,and some is soluble in cytoplasm(Becker,1994;Safi et al.,2014).Herein,the portion of proteins in water-soluble COM of C.vulgaris determined by the modified Lowry method was about 10%of dry weight(DW),which conforms with the yield of the thermal precipitation method separating about 9%of DOC.The same amount of proteinaceous DOC(9%)was acquired with the salting out method.HPSEC-DAD at 280 nm analyses showed that some organics of apparent MW 800–6000 Da remained in the COM after thermal precipitation(data not shown)indicating the presence of low-MW peptides.The presence of nitrogenous compounds,which may contain amino acids peptides,nucleotides and nucleic acids,was confirmed also by measuring TN.The N/C ratio was about 0.1.