《Table 1 Ingredients and proximate composition of basal feed (dry basis)》

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《Influence of two different dietary zinc sources in freshwater prawn Macrobrachium rosenbergii post larvae》

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1 Becosules capsules（manufactured by Pfi zer），each capsule contains thiamine mononitrate10 mg；ribofl avin 10 mg；pyridoxine hydrochloride3 mg；Vitamin B12 15μg；niacinamide 1P 100 mg；calcium pantothenate1P 50 mg；folic acid 1P 1.5 mg；biotin USP 100μg；asco

Experimental feeds were prepared using locally available feed ingredients（Table 1）.Feed ingredients were purchased from local shops.For preparation of these experimental feeds，fi shmeal and soybean meal，wheat fl our and tapioca fl our，and cod liver oil were used as protein，carbohydrate and lipid sources respectively.Also，tapioca fl our and egg albumin were used as binding agents，and vitamins（B complex with vitamin C）and Zn free mineral mix were also added.The optimum concentrations（60 mg/kg）of dietary B-Zn and Zn-NPs were selected for M.rosenbergii PL according to previous reports of Muralisankar et al.（2014，2015a）.In the present study，99.99%pure B-Zn（size 10μm）and Zn-NPs（size 50 nm）were purchased from Sigma-Aldrich Chemicals Pvt.Limited，Bangalore，India.These two diff erent sources of Zn were supplemented in the basal feeds at the concentration of 60 mg/kg separately.Feed without supplementation of Zn source was served as control.According to the method of Muralisankar et al.（2014），about 3.0±0.84 mm dia feeds were prepared and stored in plastic containers until used for feeding experiments.