《Table 3 Distant hybridization experiments of freshwater fishes (31 crosses, 25 crosses form survivi

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《Establishment and application of distant hybridization technology in fish》

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Through long-term and systematic studies that utilized freshwater fishes（2n=100 and 2n=48）as the main research objects（including common carp，crucian carp，blunt snout bream，Culter alburnus，Bleeker’s yellowtail，grass carp，silver carp，bighead carp and other commercial fishes），we conducted 31 distant crosses and obtained 25 kinds of viable offspring（Table 3），which lays a strong experimental foundation for exploring the genetic rules of distant hybridization in fish.We classified the hybridization of hybridized fish parents into two types of patterns.One pattern was that the parents of the hybridization had the same chromosome number.The other pattern was that the parents of the hybridization had different chromosome numbers.This classification coveredallhybridization types，including interspecific and intraspecific hybridization.After performing many studies regarding fish hybridization（Chen et al.，2017；He et al.，2012；He et al.，2013；Hu et al.，2012；Hu et al.，2018；Liu，2010；Liu et al.，2007b；Qin et al.，2010；Qin et al.，2014；Song et al.，2012；Wang et al.，2015a；Wang et al.，2017；Wang et al.，2018；Xiao et al.，2014；Xu et al.，2015；Zhang et al.，2014）（referring to Table 3） ，combined with previous relevant studies in our laboratory（such as the study of allotetraploid hybrids）（Liu et al.，2001） ，we obtained the genetic rules of distant hybridization in fish as described below: