《Table 1 Signifi cantly changed metabolites of eelgrass shoots following 48 h of exposure to 32℃》

  提示：宽带有限、当前游客访问压缩模式

本系列图表出处文件名：随高清版一同展现

《Photosynthetic and metabolic responses of eelgrass Zostera marina L. to short-term high-temperature exposure》

1. 获取 高清版本忘记账户？点击这里登录

1. 下载图表忘记账户？点击这里登录

The fold changes were calculated using log2 transformed data（32℃exposure/control at 16℃）.Similarity，comparison between compounds and the standard mass chromatogram；R.T.:retention time；VIP:variable importance in projection of the fi rst principal compo

For multivariate analysis of the eff ects of temperature on the metabolome of Z.marina，exploratory unsupervised PCA analysis was conducted to identify the natural relationships between the two groups.No obvious clustering was found between the two groups in the PCA；therefore，further analysis was conducted by OPLS-DA using temperature as a classifi er.The samples were better separated on the score map of OPLA-DA（Fig.3）and the parameters obtained by 7-fold cross-validation were R 2 Y=0.995 and Q 2=0.657，P CV-ANOVA=0.07.After10 row permutations of the dataset with 7-fold of cross-validation each time（Triba et al.，2015），10 Q 2values were obtained and determined to be stable，with a mean of Q 2=0.729（SD=0.03）and P CV-ANOVA=0.035（SD=0.012）.The model’s good predictability was further validated by a permutation test with R 2 Y=0.982 and Q 2=0.024 2.Based on the VIP value from the OPLA-DA mode and substances’similarity，14 diff erential metabolites with similarity>700 and VIP>1 were identifi ed as signifi cantly changed metabolites，of which 12 were known and two were unknown（Table 1）.These signifi cantly changed metabolites in eelgrass shoots were involved in sugar metabolism，the citric acid cycle and metabolism of amino acids and were summarized in a simplifi ed metabolic map（Fig.4）.