《Table 1Cancer associated fibroblasts from six different patients.》

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《Tumor–stromal cross-talk modulating the therapeutic response in pancreatic cancer》

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Freshly resected pancreas tissue was rinsed in cold RPMI 1640medium and cut into small cubes of approximately 1 mm 3.These cubes were incubated under standardized conditions and medium was changed 24 h after isolation to avoid contamination.CAFs from six different patients were isolated and cultured.Of those，four originated from an adenocarcinoma of the pancreas head，one from the papilla and one from an intrahepatic cholangiocarcinoma（Table 1）.Stromal composition and desmoplastic reaction were described in the pathological report by a pathologist specialized in pancreas.None of the six patients received neoadjuvant chemotherapy.After the isolation process，primary patient derived CAFs were grown and used for experiments between passages 3and 6.Notably，no growth factors were added and no pre-coating of culture plates was conducted.