《Table 1 Primers for the amplifications of soil bacteria, fungi, and Bacillus》

《Table 1 Primers for the amplifications of soil bacteria, fungi, and Bacillus》   提示：宽带有限、当前游客访问压缩模式
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《Soil microbial activity and community structure as affected by exposure to chloride and chloride-sulfate salts》


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Bacillus，group-selective community profiling was based on the nested PCR method of Garbeva et al.（2003）.Primers BACF and R1378 were used in the first-round PCR.Primers BACF and R1378 were used in the first-round PCR at 0.2 m M each（Table 1）.The reaction mixture also contained 1U of Hot Star Taq polymerase（Qiagen），2.5μL of 10×buffer，10 m M of each d NTP，2m L of 1/10 diluted DNA，and dd H2O to a final volume of 25μL.First round PCR used a touchdown thermos-cycling profile.Following hot-start polymerase activation（5 min at 94°C），denaturation was conducted at 95°C for 1 min，extension at 68°C for 2 min.Annealing was initially at 63°C（1 min）and reduced by 2°C per cycle until reaching 55°C.A further 21 cycles with a 55°C annealing temperature completed the PCR.Second-round PCR was used primers F968-GC and R1378 with 1:100 diluted of the first-round PCR product as template.Otherwise，the chemistry and PCR conditions were as for general bacteria.

图表编号XD0020362700    严禁用于非法目的
绘制时间2018.10.01
作者ZHANG Qianqian、Steven A WAKELIN、LIANG Yongchao、CHU Guixin
绘制单位College of Life Science, Shaoxing University、Department of Resources and Environmental Science, College of Agriculture、the Key Laboratory of Oasis Eco-agriculture of the Xinjiang Production and Construction Corps, Shihezi University、College of Resources a
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