《Table 1 Disinfection situation of seeds of Betula microphylla Bunge var.paludosa》

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《Tissue Culture of Betula microphylla Bunge var. paludosa》

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Note:The seeds in the treatment groups were further soaked in sterile water for 1 h and disinfected with sodium hypochlorite for 15 min after treated as describedin the table.

A total of nine disinfection treatments were designed for the seeds of B.microphylla（Table 1）.The seeds were then soaked in sterile water for 1 h and disinfected with 1%sodium hypochlorite for 15 min.As shown in Table 1，when the 300 seeds were soaked in 75%alcohol for 30 s and disinfected with 5%sodium hypochlorite for20 min，none of them was infected with bacterial，with infection rate of zero，indicating good treatment effect.